The head coach also ruled out in-form forward Iago Aspas, but called up Borjo Iglesias and Nick Williams without matches.
The upcoming games will be Spain’s last before Luis Enrique picks his World Cup squad, so all eyes have been on whether former captain Ramos in particular will return.
Ramos won a record 180 caps for Spain but may have played his last game for La Roja unless Luis Enrique changes his mind before November.
The door is left ajar, but time is running out for Ramos and Aspas.
“These players played in the national team. They can return to the national team without any doubts,” Luis Enrique said.
It could be a different story for Fati, who has been favored when fit and is returning to his best form at Barcelona after an injury-interrupted 2021-22 campaign.
Fati has yet to start a LaLiga game this season but has scored two goals and provided two assists off the bench in just 120 minutes of action.
Luis Enrique said such limited action is telling at this stage, adding: “Hopefully we’ll see Ansa at his best again, but I don’t see him on the list today. He is in the process of gaining confidence and improving after not playing for a long time.”
Speaking at the press conference, Enrique said: “Every time the team list comes out, the attention is drawn to those who are not there, but I prefer to talk about those who are. I have 25 players who I think are the best for this match.’
Athletic Bilbao’s Williams, whose brother Inaki recently switched his football allegiance from Spain to Ghana, was described by Luis Enrique as a “pure winger” and a player the coaching staff wanted to see up close.
Luis Enrique already knows Betis forward Borja, having previously coached him at Celta Vigo, and said he appreciated the 29-year-old’s always-smiling style on the pitch. He already has four goals in La Liga this season.
Aspas can probably make plans for November and December that do not involve Qatar 2022. Celta Vigo’s veteran striker has been a standout performer in LaLiga and has already scored five goals in the competition this season, just one short of top scorer Robert Lewandowski.
Even more remarkably, Aspas has achieved his goals despite having an Expected Goals (xG) of just 1.4, suggesting that he is well above what was expected of him.
However, the 35-year-old last played for Spain in 2019.
Spain take on Switzerland at La Ramoreda in Zaragoza on September 25 (AEST), before taking on Portugal in Braga three days later.
Spain national team: Unai Simon (Athletic Bilbao), Robert Sanchez (Brighton and Hove Albion), David Raya (Brentford); Pau Torres (Villarreal), Jordi Alba (Barcelona), Jose Gaia (Valencia), Hugo Guillamon (Valencia), Diego Llorente (Leeds United), Eric Garcia (Barcelona), Cesar Azpilicueta (Chelsea), Dani Carvajal (Real); Sergio Busquets (Barcelona), Rodri (Manchester City), Pedri (Barcelona), Koke, Gavi (Barcelona), Carlos Soler (Paris Saint-Germain), Marcos Llorente (Atletico Madrid) ); Pablo Sarabia (Paris Saint-Germain), Nico Williams (Atletico Bilbao), Marco Asensio (Real Madrid), Alvaro Marato (Atletico Madrid), Borja Iglesias (Real Betis), Jeremy Pino (Villarreal), Ferran Torres (Barcelona).